ISO 19574:2022 pdf – Footwear and footwear components — Qualitative test method to assess antifungal activity (growth test)

ISO 19574:2022 pdf – Footwear and footwear components — Qualitative test method to assess antifungal activity (growth test)

ISO 19574:2022 pdf – Footwear and footwear components — Qualitative test method to assess antifungal activity (growth test).
1 Scope This document specifies a test method (growth test) for the qualitative evaluation of the antifungal activity of footwear and footwear components exposed to the action of filamentous micro-fungi. This document is only applicable to footwear and components that claim to have antifungal (antimycotic) or antimicrobial treatment effects. 2 Normative references The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media ISO 16187, Footwear and footwear components — Test method to assess antibacterial activity ISO 19952, Footwear — Vocabulary 3? Terms? and? definitions For the purposes of this document, the terms and definitions given in ISO 19952 and the following apply. ISO and IEC maintain terminology databases for use in standardization at the following addresses: — ISO Online browsing platform: available at https:// www .iso .org/ obp — IEC Electropedia: available at https:// www .electropedia .org/ 3.1 antifungal activity antimycotic activity efficacy of a material or finish used to prevent or mitigate the growth of micro-fungi, to reduce the number of micro-fungi or to kill micro-fungi 3.2 control specimen material identical to the test material but without antifungal treatment
4 Principle The test and control specimens of footwear are inoculated with a mixed spore suspension of selected mould test strains or with a single test strain according to the specific claim. Antifungal performance is qualitatively determined by visual assessment of fungal growth after specified or agreed incubation period. 5 Safety Handling of microorganisms that are potentially hazardous requires a high degree of technical competence and can be subject to current national legislation and regulations. Only personnel trained in microbiological techniques should carry out such tests. NOTE Refer to country-specific codes of practice for personal hygiene, disinfection and sterilization. The persons who perform the test should consult IEC 60068-2-10:2005+AMD1: 2018, Annex A and ISO 7218. 6 Apparatus 6.1? General Disposable apparatus is an acceptable alternative to re-usable glassware and plastic if it has suitable specifications. The usual microbiological laboratory equipment in accordance with ISO 7218 and in particular the following shall be used. 6.2? Biological? safety? cabinet. 6.3? Microbiological? incubator, capable of maintaining a temperature of (28 ± 2) °C and a relative humidity of (85 ± 5) %. 6.4? Autoclave,? capable of maintaining a temperature of (121 ± 2) °C and a pressure of (103 ± 5) kPa, for wet sterilization, used in accordance with ISO 7218.
6.13? Balance, capable of weighing to ±0,01 g. 6.14? Spectrophotometer, capable of measuring at 500 nm to 700 nm wavelength, or McFarland’s nephelometer. 6.15? Petri? dishes, that have been sterilized, made of glass or plastic, in diameter sizes of 90 mm to 100 mm or 55 mm to 60 mm. 6.16? Pipettes, having the most suitable volume for each use. 6.17? Grid, for evaluation of fungal growth, each square has a size of 5 mm × 5 mm, or that divides the surface of the test specimen into 100 equal sized squares. 7 Reagents and culture medium 7.1? General The reagents used in the tests shall be of analytical quality and/or suited for microbiological purposes. Dehydrated products available on the commercial market should be used in the preparation of the culture media. The manufacturer’s instructions for the preparation of these products should be strictly followed. The preparation, production and performance testing of culture media shall be in accordance with ISO 11133. 7.2? Water The water used in the tests shall be analytical-grade water for microbiological media preparation, which is freshly distilled and/or ion-exchanged and/or ultra-filtered and/or filtered with RO (Reverse Osmosis). It shall be free from all toxic or microorganism inhibitory substances.

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