BS EN ISO 7541:2020 pdf – Spices and condiments — Spectrophotometric determination of the extractable colour in paprika.
1 Scope This document specifies a test method to determine the extractable colour in paprika by measuring the absorbance of an acetone extract of the sample. It is applicable to ground paprika in every presentation (sweet, hot, smoked, etc). 2 Normative references There are no normative references in this document. 3? Terms? and? definitions For the purposes of this document, the following terms and definitions apply. ISO and IEC maintain terminological databases for use in standardization at the following addresses: — ISO Online browsing platform: available at https://www.iso.org/obp — IEC Electropedia: available at http://www.electropedia.org/ 3.1 extractable colour soluble matter (extract) in acetone, determined according to the procedure described in this document 3.2 paprika product obtained by grinding the dry and mature fruits of Capsicum annuum L. or Capsicum frutescens L. 4 Principle Extraction from the test sample using acetone. Measurement of the absorbance of the solution obtained using a spectrophotometer at a wavelength of 460 nm. 5 Reagents All reagents shall be of a recognized analytical grade. 5.1 Acetone. 6 Apparatus Usual laboratory equipment and, in particular, the following. 6.1 Analytical balance, capable of weighing with a resolution of 0,1 mg. 6.2 Graduated pipette, of 10 ml capacity, class A.
The capsanthin concentration C, in g/kg, of the undried sample is determined from the extractable colour, E, in ASTA units, using Formula (2): C = E × 0,169 (2) NOTE The precision of the method was established in accordance with ISO 5725-2 by an international interlaboratory test. The results of the interlaboratory tests are given in Annex A. 11 Expression of results The results of the extractable colour shall be expressed in ASTA units and shall be reported to the nearest whole number. 12 Test report The test report shall contain at least the following information: a) the method used, including a reference to this document, i.e. ISO 7541:2020; b) all information necessary for the complete identification of the sample; c) the results obtained, including a reference to the clause which explains how the results were calculated; d) any deviations from the procedure; e) any unusual features observed; f) all operation details not specified or regarded as optional, as well as any incidents which may have influenced the results; g) the date of the test.